Evaluation of the Effect of Vitamin E on Reproductive Parameters in Morphine-Treated Male Mice

Document Type : Original Article


1 Masters student, Department of Anatomical Sciences, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran

2 Department of Anatomical Sciences, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran

3 Social Determinants of Health Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran



Background: Morphine is a narcotic pain reliever that is prescribed to reduce postoperative pain and can produce reactive oxygen 
species (ROS). Therefore, it can have negative effects on spermatogenesis and male fertility. Vitamin E is an effective antioxidant 
which plays an important role in membrane lipid peroxidation due to increased ROS. The present study aimed to evaluate the 
effects of vitamin E and morphine on sperm parameters, level of malondialdehyde (MDA), and diameter of seminiferous tubules in 
morphine-treated mice. 
Methods: In this experimental study, 80 mice were divided into ten groups (n=8) including control, normal saline, vehicle, morphine, 
various doses of vitamin E (100, 200, 300 mg/kg), and morphine plus vitamin E (100, 200, 300 mg/kg) groups. The groups were 
followed up for 30 consecutive days. Sperm parameters, testis weight, the diameter of seminiferous tubules, and the level of MDA 
were analyzed and compared.
Findings: Data analysis showed seminal parameters decreased significantly (excluding sperm count) and there was an increase in 
the level of MDA in morphine-treated mice compared with the normal saline group (P<0.05). Administration of E100 to morphinetreated mice did not show a significant difference in the evaluated parameters compared with the morphine group. However, E200 
and E300 significantly reduced MDA and improved sperm parameters (P≤0.05).
Conclusion: The results showed co-administration of vitamin E in high doses (200 & 300) could prevent the deleterious effects of 
morphine on some reproductive parameters and decrease the level of MDA in morphine-treated mice.


Katayoon Arjmand: (Google Scholar) (PubMed)

Erfan Daneshi: (Google Scholar) (PubMed)

Soheila Pourmasumi: (Google Scholar) (PubMed)

Fardin Fathi: (Google Scholar) (PubMed)

Sherko Naseri: (Google Scholar) (PubMed)

Parvin Sabeti: (Google Scholar) (PubMed)


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