Kerman University of Medical Sciences

Document Type: Original Article

Authors

1 Department of Exercise Physiology, School of Sport Sciences, Urmia University, Urmia, Iran

2 Neuroscience Research Center, Institute of Neuropharmacology AND Cardiovascular Research Center, , Kerman University of Medical Sciences, Kerman, Iran

3 Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran

4 Physiology Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran

5 Pathology and Stem Cell Research Center, Kerman University of Medical Sciences, Kerman, Iran

6 Neuroscience Research Center, Institute of Neuropharmacology AND Department of Physiology and Pharmacology, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran

Abstract

Background: The use of waterpipe tobacco smoking (WTS) is on the rise throughout the world, especially
among young people and even athletes. There is a belief among consumers that exercise prevents the harmful
effects of hookah smoke on the body. We examined this belief by evaluation of lung injury following to
concurrent WTS and swimming endurance training in male Wistar rats.
Methods: Animals were randomly divided to sedentary control (CTL) group, exercise training group
(Ex group), sedentary WTS (S) group, and exercise plus WTS (S + Ex) group.
Findings: 8 weeks of WTS was associated with significant increase in serum level of cotinine, lung damage,
reduction in alveolar number AN/SA (mm2) and increase in malondialdehyde (MDA) level of lung tissue.
Combination of exercise with WTS significantly decreased these negative effects; however, it could not fully
protect the lung from smoking damage. Waterpipe smoking (WPS) also significantly increased the proinflammatory cytokines of lung tissue such as tumor necrosis factor alpha (TNF-α) (P < 0.001), interleukin 1
beta (IL-1β) (P < 0.010), and IL-6 (P < 0.050) in comparison with CTL group. Exercise training to some
degree reduced the levels of pro-inflammatory cytokines and increased the level of IL-10 as an antiinflammatory IL and glutathione peroxidase (GPX) activity in animals exposed to WTS.
Conclusion: It is suggested that combination of mild to moderate exercise with WTS may attenuate the
hookah smoking-induced lung damage. This effect partly is mediated through balancing of pro/antiinflammatory and redox systems


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