Kerman University of Medical Sciences

Document Type: Original Article(s)

Authors

1 Professor, Physiology Research Center, Institute of Neuropharmacology, Department of Biochemistry, School of Medicine, Kerman ‎University of Medical Sciences, Kerman, Iran

2 PhD Student, Molecular Biology Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

3 Associate Professor, Physiology Research Center, Institute of Neuropharmacology, Department of Physiology, School of Medicine, ‎Kerman University of Medical Sciences, Kerman, Iran

4 Professor, Physiology Research Center, Institute of Neuropharmacology, Department of Physiology, School of Medicine, Kerman ‎University of Medical Sciences, Kerman, Iran

5 Research Center for Modeling in Health, Institute for Futures Studies in Health, Kerman University of Medical Sciences, Kerman, Iran

6 Professor, Department of Immunology, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

7 Assistant Professor, Department of Immunology, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

Abstract

Background: The risk of infectious, autoimmune and immunodeficiency diseases and cancers rise in opioid addicts due to changes in innate and acquired immune responses. Three types of opioid receptors (К،δ،μ) are expressed on the surface of lymphocytes and mononuclear phagocytes. The present study was designed to examine the effects of different concentrations of opium on the secretion of some cytokines produced by lymphocyte cells. Methods: Jurkat cells were exposed to different concentrations of opium for periods of 6, 24 and 72 h in cell culture medium. The amount of interleukin-6 (IL-6), interferon-γ (IFN-γ) and transforming growth factor-b (TGF-β) were then measured using enzyme-linked immunosorbent assay (ELISA) method. Findings: The results showed that opium increases the secretion of IL-6 in different concentration of opium in 6 h. The amount of IFN-γ decreased in 6 h and increased in 24 h significantly compared with control. On the other hand, opium had an inhibitory effect on the TGF-β secretion in 6, 24 and 72 h. Conclusion: Overall, the study showed that opium stimulates pro-inflammatory and suppressed anti-inflammatory cytokine secretion in Jurkat cells. This may account for the negative effect of opium on the immune system leading to chronic inflammation and a base for many disorders in opium addicts.

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